Pseudomonas Fluorescens Manufacturer & Exporter | Plant Growth Promoters | Microbial Species

Pseudomonas fluorescens

Pseudomonas fluorescens suppresses soil-borne pathogens, produces antibiotics and siderophores, enhances nutrient availability, improves root growth and disease resistance.

Strength

1 x 10⁸ CFU per gram / 1 x 10⁹ CFU per gram / 1 x 10¹⁰ CFU per gram

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Benefits

Promotes plant growth through siderophore production

Pseudomonas fluorescens produces siderophores, which chelate iron and make it available to plants, thereby enhancing plant growth.

Controls soil-borne pathogens

Effectively suppresses the growth of various soil-borne pathogens such as Fusarium, Pythium, and Rhizoctonia, reducing disease incidence in plants.

Enhances nutrient availability in the rhizosphere

Improves the availability of nutrients like phosphorus and zinc, facilitating better nutrient uptake by plants for improved growth.

Stimulates root development

Stimulates root elongation and proliferation, leading to enhanced nutrient absorption and overall plant health.

Scientific References

Haas, D., & Défago, G. (2005). Biological control of soil-borne pathogens by fluorescent pseudomonads. Nature Reviews Microbiology, 3(4), 307–319. https://doi.org/10.1038/nrmicro1129
Raaijmakers, J. M., et al. (2002). Antibiotic production by bacterial biocontrol agents. Antonie van Leeuwenhoek, 81(1), 537–547. https://doi.org/10.1023/A:1020501420831
Weller, D. M. (2007). Pseudomonas biocontrol agents of soilborne pathogens: Looking back over 30 years. Phytopathology, 97(2), 250–256. https://doi.org/10.1094/PHYTO-97-2-0250
Chin-A-Woeng, T. F. C., et al. (2003). Phenazines and their role in biocontrol by Pseudomonas bacteria. New Phytologist, 157(3), 503–523. https://doi.org/10.1046/j.1469-8137.2003.00665.x
Glick, B. R. (2012). Plant growth-promoting bacteria: Mechanisms and applications. Scientifica, 2012, 963401. https://doi.org/10.6064/2012/963401
Singh, A., & Ward, O. P. (2004). Biodegradation and Bioremediation. Springer. ISBN: 978-3-540-21008-2

Mode of Action

1. Pathogen Suppression

Siderophore Production: Chelates iron, making it unavailable to phytopathogens like Fusarium, Pythium, and Rhizoctonia.
Antibiotic Secretion: Produces metabolites like 2,4-diacetylphloroglucinol (DAPG), phenazine-1-carboxylic acid (PCA), and pyoluteorin that inhibit fungal growth.
Competitive Exclusion: Rapid colonization of the rhizosphere prevents establishment of pathogenic organisms.
Induced Systemic Resistance (ISR): Triggers host plant defenses akin to acquired immunity via salicylic acid and jasmonic acid pathways.

2. Plant Growth Promotion

Phytohormone Synthesis: Produces indole-3-acetic acid (IAA) which enhances root development and nutrient absorption.
Phosphorus Solubilization: Converts insoluble phosphates to bioavailable forms via organic acid secretion.
Nitrogen Fixation & Nutrient Mobilization: Improves uptake of nitrogen, potassium, and trace elements.

3. Bioremediation

Hydrocarbon Degradation: Utilizes oxygenases and peroxidases to break down complex organic compounds like crude oil and pesticides.
Heavy Metal Detoxification: Bioaccumulates and transforms metals (e.g., cadmium, nickel) through redox reactions, reducing their phytotoxicity.

Additional Info

Formulation Types
- Liquid Suspension and Talc-Based Powder formulations are available for diverse application methods including seed treatment, soil application, and irrigation-based delivery systems.
Shelf Life
- Stable for up to 1 year from the date of manufacturing under recommended storage conditions (cool, dry place away from direct sunlight and moisture).
Storage Guidelines
- Store in original, sealed packaging at room temperature (preferably 4–30°C).
- Avoid exposure to high humidity or freezing conditions.
- Shake well before use in case of sedimentation in liquid formulations.
Compatibility
- Compatible with most organic manures, microbial consortia, and biostimulants.
- Avoid simultaneous use with strong chemical fungicides. Apply in staggered intervals if necessary.
Packing
- Tailor-made packaging available to meet customer-specific requirements, including bulk and retail formats.
- Options include 250 g, 500 g, 1 kg for powder and 250 mL, 500 mL, 1 L for liquid, with private labelling support available on request.
Regulatory & Safety Compliance
- Complies with organic farming regulations (NPOP, USDA-NOP).
- Safe for applicators, animals, soil microfauna, and non-target organisms.
- Non-toxic, non-pathogenic, and environmentally sustainable.

Dosage & Application

Agricultural Applications

Seed Treatment
- Preparation: Mix 10 g of Pseudomonas fluorescens powder or 10 mL of liquid formulation with 10 mL of a 10% jaggery or sugar solution per kg of seed.
- Method: Coat seeds uniformly and allow to shade-dry for 30 minutes before sowing.
- Purpose: Protects seedlings from early soil-borne infections and enhances early root development.
Seedling Root Dip
- Preparation: Dilute 10 g or 10 mL of the formulation per liter of water.
- Method: Immerse seedlings in the suspension for 20–30 minutes prior to transplantation.
- Purpose: Establishes beneficial microbial populations in the rhizosphere at early growth stages.
Soil Application
- Preparation: Mix 2–5 kg of P. fluorescens with 100–200 kg of compost or well-decomposed farmyard manure per acre.
- Method: Apply to soil before sowing or during active root zone development.
- Frequency: Apply 2–3 times per cropping season for persistent soil colonization.
- Purpose: Suppresses soil-borne pathogens and enhances nutrient cycling.
Drip Irrigation / Foliar Spray
- Preparation: Mix 1–2 L per acre in sufficient water for irrigation systems or foliar sprays.
- Use Case: Targeted during high disease pressure or as a maintenance dose in precision farming systems.

Environmental Remediation Applications

Apply in concentrations of 10⁶–10⁸ CFU/mL to contaminated soils or water bodies.
Co-inoculate with organic substrates to stimulate microbial degradation of hydrocarbons and heavy metals.
Periodic re-application may be required depending on pollutant load and environmental conditions.

Industrial Applications

Dosage optimized based on bioreactor volume and desired metabolite yield (e.g., biosurfactants).
Integrated into wastewater treatment plants at inoculation rates sufficient to reduce BOD/COD and degrade complex pollutants.

FAQ

What are the main applications of Pseudomonas fluorescens?

It is widely used in agriculture for plant growth promotion and suppression of soil-borne diseases. It also supports environmental cleanup through pollutant degradation and has applications in biotechnology for biosurfactant and biopolymer production.

How does Pseudomonas fluorescens help crops resist diseases?

It protects plants by producing siderophores that limit pathogen iron availability, secreting antimicrobial compounds, and inducing systemic resistance within plants, strengthening their natural defense system.

Is Pseudomonas fluorescens effective under abiotic stress?

Yes, it improves plant tolerance to drought, salinity, and heavy metal stress by enhancing root function and reducing oxidative damage, leading to better plant resilience.

Can it be used in organic farming?

Yes, it is widely accepted in organic farming systems as a bio-based solution for crop protection and soil health improvement.

Is Pseudomonas fluorescens safe for the environment?

It is non-pathogenic, environmentally safe, does not accumulate in higher organisms, and supports beneficial microbial balance in soil ecosystems.

Where is Pseudomonas fluorescens commonly found in nature?

It naturally exists in soil, water, and plant root zones, where it plays a key role in nutrient cycling and plant-microbe interactions.

Reference: Natural habitat of Pseudomonas fluorescens

What are the oxygen requirements of Pseudomonas fluorescens?

It is an aerobic bacterium that requires oxygen for growth and metabolic activity, commonly thriving in oxygen-rich soil and rhizosphere environments.

How long does Pseudomonas fluorescens persist in soil?

It can survive and colonize the rhizosphere for several weeks under suitable conditions, although periodic reapplication is often recommended for sustained effectiveness.

Can it be combined with other microbial products?

Yes, it is commonly compatible with beneficial microbes such as Bacillus, Trichoderma, and mycorrhizal fungi, though compatibility with chemical treatments should be evaluated carefully. Compare: Pseudomonas vs Trichoderma

Does it affect pollinators or beneficial insects?

No, it is considered safe for bees, earthworms, and other beneficial organisms in agricultural ecosystems.

How should Pseudomonas fluorescens be stored?

It should be stored in a cool, dry, and shaded environment, protected from direct sunlight and extreme temperatures to maintain microbial viability.

How is it better than synthetic agrochemicals?

It improves long-term soil health, reduces chemical dependency, and helps avoid pesticide resistance while maintaining sustainable crop productivity.

Reference: Pseudomonas fluorescens and crop health