Bacillus Amyloliquefaciens Manufacturer & Exporter | Plant Growth Promoters | Microbial Species

Bacillus amyloliquefaciens

Bacillus amyloliquefaciens, produces plant growth hormones, suppresses pathogens with enzymes, acts as biofertilizer and biopesticide, improves soil fertility, safe for non-target species and humans.

Strength

1 x 10⁸ CFU per gram / 1 x 10⁹ CFU per gram / 1 x 10¹⁰ CFU per gram

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Benefits

Suppresses plant pathogens

It produces antibiotics, siderophores, and other metabolites that inhibit the growth of plant pathogens like fungi, bacteria, and nematodes.

Enhances nutrient uptake

Facilitates nutrient uptake by solubilizing phosphates and micronutrients in the soil, improving nutrient availability to plants for better growth and health.

Improves soil fertility

Enhances soil fertility by promoting nutrient cycling, particularly nitrogen and phosphorus, through enzymatic breakdown of organic matter.

Produces plant growth hormones

Bacillus amyloliquefaciens synthesizes and releases plant growth-promoting hormones (auxins, cytokinins, gibberellins), which stimulate plant growth.

Scientific References

Mode of Action

Rhizosphere Colonization and Biofilm Formation
- Rapid chemotaxis toward root exudates (sugars, amino acids) establishes populations of 10^7–10^8 CFU/g soil within 5–7 days.
- Exopolysaccharide-mediated biofilm on root surfaces enhances persistence and protects cells from desiccation and predators.
Nutrient Mobilization
- Phosphate Solubilization: Secretion of organic acids (gluconic, citric, oxalic) and phosphatases lowers soil pH and liberates insoluble inorganic phosphates for plant uptake.
- Siderophore Production: High-affinity siderophores chelate Fe^3+ and deliver iron to roots, correcting micronutrient deficiencies and suppressing iron-dependent pathogens.
Phytohormone Synthesis
- Indole-3-Acetic Acid (IAA) Biosynthesis: Tryptophan-dependent pathways generate 5–20 µg IAA/mL, stimulating root hair formation, lateral root branching, and root elongation.
- Cytokinin Production: Low-level zeatin and kinetin analogs (0.2–0.5 µg/mL) promote cell division in meristematic tissues, balancing shoot-to-root growth.
Enhanced Nitrogen Acquisition
- Nodulation Induction in Legumes: Production of lipo-oligosaccharides and phytohormones upregulates nodulation (Nod) genes in rhizobia, increasing nodule number by up to 50% and boosting biological N₂ fixation.
Pathogen Suppression
- Antibiotic Secondary Metabolites: Nonribosomal lipopeptides (iturins, fengycins, surfactins) disrupt fungal cell membranes, reducing spore germination and hyphal growth by >80% in vitro.
- Hydrolytic Enzymes: Chitinases, β-1,3-glucanases, proteases degrade pathogen cell walls, providing broad-spectrum biocontrol.
Induced Systemic Resistance (ISR)
- Elicitation of Plant Defense: Flagellin fragments and cyclic lipopeptides trigger jasmonic acid and ethylene pathways, priming systemic resistance against bacteria, fungi, and insects.
Stress Alleviation
- ACC Deaminase Activity: Degradation of plant ACC (ethylene precursor) lowers stress ethylene levels, maintaining root growth under drought and salinity.
- Osmolyte Accumulation: Upregulation of proline and glycine betaine in plants under stress enhances cell turgor and membrane stability.
Biofilm-Mediated Detoxification
- Heavy Metal Chelation: Surface-bound extracellular polymeric substances sequester cadmium, lead, and arsenic, reducing phytotoxicity and improving plant growth in contaminated soils.

Additional Info

Recommended Crops: Cucurbits, Grapes, Apple, Peas, Beans, Tomato, Pulses, Cumin, Chilies, Coriander, Mango, Ber, Peas, Strawberry, Medicinal and Aromatic crops, and Roses

Compatibility: Compatible with Bio Pesticides, Bio Fertilizers, and Plant growth hormones but not with chemical fertilizers and chemical pesticides.

Shelf Life: Stable within 1 year from the date of manufacturing.

Packing: We offer tailor-made packaging as per customers' requirements.

Dosage & Application

Wettable Powder: 1 x 10⁸ CFU per gram

Foliar Application

1 Acre dose: 3-5 kg
1 Ha dose: 7.5 - 12.5 kg

Soil Application (Soil drench or Drip irrigation)

1 Acre dose: 3-5 kg
1 Ha dose: 7.5 - 12.5 kg

Soil Application for Long duration crops / Orchards / Perennials

1 Acre dose: 3-5 kg
1 Ha dose: 7.5 - 12.5 kg
Apply 2 times in 1 Year: Before onset of monsoon and after monsoon

Seed Dressing

1 Kg seed: 1g Bacillus Amyloliquefaciens + 10 g crude sugar

Foliar Application for Long duration crops / Orchards / Perennials

1 Acre dose: 3-5 kg
1 Ha dose: 7.5 - 12.5 kg
Apply 2 times in 1 Year: Before onset of monsoon and after monsoon

Soluble Powder

Foliar Application

1 Acre dose: 1 kg
1 Ha dose: 2.5 kg

Soil Application (Soil drench or Drip irrigation)

1 Acre dose: 1 kg
1 Ha dose: 2.5 kg

Soil Application for Long duration crops / Orchards / Perennials

1 Acre dose: 1 kg
1 Ha dose: 2.5 kg
Apply 2 times in 1 Year: Before onset of monsoon and after monsoon

Seed Dressing

1 Kg seed: 1g Bacillus Amyloliquefaciens + 10 g crude sugar

Foliar Application for Long duration crops / Orchards / Perennials

1 Acre dose: 1 kg
1 Ha dose: 2.5 kg
Apply 2 times in 1 Year: Before onset of monsoon and after monsoon

Soil Application Method

Mix Bacillus Amyloliquefaciens at recommended doses in sufficient water and drench soil at early leaf stage / 2-4 leaf stage / early crop life cycle.
Drip Irrigation: If there are insoluble particles, filter the solution and add to drip tank.
Long duration crops / Perennial / Orchard crops: Dissolve Bacillus Amyloliquefaciens at recommended doses in sufficient water and apply as a drenching spray near root zone twice a year. It is recommended to have first application before the onset of the main monsoon / rainfall / spring season and second application after the main monsoon / rainfall / autumn / fall season.

Seed Dressing Method

Mix Bacillus Amyloliquefaciens with crude sugar in sufficient water to make a slurry and coat seeds. Dry in shade and sow / broadcast / dibble in the field. Do not store treated / coated seeds more than 24 hours.

Foliar Application Method

Foliar application to be done at early disease incidence. 1-2 follow-up sprays to be done at weekly intervals.
Mix Bacillus Amyloliquefaciens at recommended doses in sufficient water and spray on foliage / fruit / plant. Apply twice a year for long duration crops. It is recommended to have first application before the onset of the main monsoon / rainfall / spring season and second application after the main monsoon / rainfall / autumn / fall season.

Note: Do not store Bacillus Amyloliquefaciens solution for more than 24 hours after mixing in water.

FAQ

What is Bacillus amyloliquefaciens?

Bacillus amyloliquefaciens is a beneficial soil-borne bacterium that forms hardy endospores. It colonizes plant roots and promotes growth and health through multiple mechanisms.

How does B. amyloliquefaciens colonize plant roots?

It moves toward root exudates (sugars, amino acids), attaches via biofilm formation, and reaches 10⁷–10⁸ CFU per gram of root zone within a week.

What nutrients does it mobilize?

Phosphate solubilization: secretes organic acids and enzymes to convert insoluble phosphorus into plant-available forms

Siderophore production: chelates iron from soil, making it accessible to plants and starving pathogens

Which phytohormones does it produce?

Indole-3-acetic acid (IAA) to stimulate root elongation and branching Cytokinin analogs to promote cell division in shoots and improve shoot-root balance.

How does it enhance nitrogen fixation in legumes?

It secretes signals (lipo-oligosaccharides and phytohormones) that induce nod gene expression in rhizobia, increasing nodule number by up to 50% and boosting atmospheric N₂ fixation.

What biocontrol activities does it offer?

It produces antimicrobial lipopeptides (iturins, fengycins, surfactins) that disrupt fungal and bacterial membranes, and secretes chitinases to degrade fungal cell walls, suppressing soil-borne pathogens.

Can it induce systemic resistance (ISR)?

Yes. Its molecules (flagellin peptides, cyclic lipopeptides) trigger jasmonate and ethylene pathways in plants, priming systemic defenses against a broad spectrum of pests and diseases.

Does it help plants under drought or salinity stress?

B. amyloliquefaciens expresses ACC deaminase, which breaks down the ethylene precursor ACC, reducing stress-induced ethylene levels. It also induces osmolyte accumulation (proline, glycine betaine) to improve osmotic balance.

How is it applied in the field?

Commonly as a seed coating (10⁶–10⁸ CFU per seed) or soil drench.

Formulations use carriers like peat or vermiculite. Optimal soil moisture and pH improve establishment.

Is B. amyloliquefaciens safe?

Yes. It is generally recognized as safe, non-pathogenic to humans and animals, and does not leave harmful residues. Standard quality control ensures strain purity and efficacy.