Pseudomonas putida Manufacturer & Exporter | Phosphorous Solubilizing Bacteria | Microbial Species

Pseudomonas putida: Beneficial Rhizobacteria for Plant Growth and Phosphorus Availability

Pseudomonas putida is a plant growth-promoting rhizobacterium with applications in crop nutrition, root development and soil management. Its agricultural importance centres on phosphorus mobilization and beneficial interactions in the rhizosphere—the biologically active zone surrounding plant roots. These functions make it a valuable component of biological crop nutrition and integrated nutrient-management programs. IndoGulf BioAg supplies P. putida in formulations ready for field application, with bulk supply, custom formulation and private-label options.

Strength

1 x 10⁸ CFU per gram / 1 x 10⁹ CFU per gram / 1 x 10¹⁰ CFU per gram

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Benefits

Scientific References

Pseudomonas putida in Agriculture: Scientific Evidence

1. Phosphate Solubilization and Plant Growth — Pandey et al. (2006)

This study demonstrated that Pseudomonas putida B0 solubilized tricalcium phosphate in laboratory culture, accompanied by a decrease in medium pH. In greenhouse maize experiments, inoculation significantly increased plant biomass. The findings connect phosphate-solubilizing activity with plant-growth responses under controlled conditions.

Current Microbiology, 53, 102–107.

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2. Root Development and Phosphorus Nutrition — Srivastava & Srivastava (2020)

Research with P. putida MTCC 5279 examined root development and plant responses to phosphorus limitation and salinity in Arabidopsis thaliana. Inoculation influenced lateral-root development, hormone-related signalling and stress physiology. This study provides insight into the interaction between beneficial bacteria, root architecture and nutrient acquisition.

Scientific Reports, 10, 5855.

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3. Plant Performance Under Salinity — Alhoqail (2024)

In greenhouse-grown mustard, P. putida RT12 improved growth, water status, chlorophyll content and nutrient uptake under salinity treatments. The study investigated ACC-deaminase activity and reported an improved potassium-to-sodium balance in inoculated plants, supporting its relevance to research on microbial management of salt stress.

Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 52(1), 13550.

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4. Siderophore Production and Iron Acquisition — Daura-Pich et al. (2020)

Using functional assays, chemical analysis and genomic investigation, the researchers demonstrated pyoverdine production in P. putida B2017. Pyoverdine is an iron-binding siderophore involved in microbial iron acquisition. The study helps explain how bacterial metabolites influence access to iron and interactions with surrounding microorganisms.

FEMS Microbiology Letters, 367(9), fnaa075.

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5. Irrigation Delivery and Combined Microbial Application — Quello et al. (2026)

This field study evaluated irrigation delivery of P. putida P3 together with Bacillus subtilis BacF in quinoa under two mineral-fertilization levels. It provides a practical example of incorporating microbial inoculants into an irrigated crop program. The combined treatment influenced biomass and grain components; the results section reported no significant inoculation main effect on commercial grain yield.

Frontiers in Agronomy, 8, 1733699.

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6. Molecular Basis of Plant-Growth-Associated Functions — Chaudhry et al. (2013)

Genome sequencing of P. putida MTCC5279 identified genes associated with auxin production, siderophores, phosphatases and ACC deaminase. These findings provide molecular context for investigating nutrient mobilization, plant–microbe interactions and stress-related functions. Functional assays and plant trials establish how these genetic capabilities are expressed in practice.

Genome Announcements, 1(4), e00560-13.

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These publications describe the named research strains and experimental conditions. Commercial performance depends on the strain, formulation, crop and application program.

Mode of Action

1. Mineral Phosphate Solubilization

Soil can contain phosphorus that is difficult for roots to access. Phosphate-solubilizing P. putida strains can acidify their immediate surroundings and release phosphate from poorly soluble mineral compounds.

Research with strain B0 demonstrated tricalcium phosphate solubilization alongside a fall in culture-medium pH. This explains an important pathway through which selected strains can contribute to phosphorus mobilization. [1]

2. Root Development and Plant Signalling

Some strains produce indole-3-acetic acid (IAA), an auxin associated with plant growth. Their interaction with the plant can also alter signalling pathways involved in root development.

Research with P. putida MTCC 5279 in Arabidopsis thaliana reported changes in lateral roots, root hairs and hormone-related responses. This provides a biological basis for investigating the organism in root-development programs. [2]

3. Siderophore Production and Iron Acquisition

Certain strains produce siderophores: compounds that bind iron and help bacteria acquire it from their surroundings. Pyoverdine production has been demonstrated in strain B2017.

This activity influences microbial access to iron and interactions within the root-zone community. It is distinct from supplying iron fertilizer directly to the crop. [4]

4. Plant Responses to Salinity

Selected strains possess traits associated with improved plant performance under salt stress. One example is ACC deaminase, an enzyme that breaks down a precursor of ethylene, a plant hormone involved in stress responses.

In greenhouse research, ACC-deaminase-producing P. putida RT12 improved growth and several physiological measures in salt-stressed mustard. These findings support further development of strain-specific stress-management applications. [3]

From Biological Activity to Agricultural Relevance

Biological activity	Agricultural relevance
Mineral phosphate solubilization	Mobilization of poorly soluble phosphorus
Growth-associated metabolites and plant signalling	Changes in root architecture and development
Siderophore production	Microbial iron acquisition and root-zone interactions
Stress-related microbial traits	Potential support for plant performance under salinity

Additional Info

Compatibility and Crop Programs

Mineral Fertilizers

P. putida can form part of an integrated crop nutrition program. The objective is to combine appropriate nutrient supply with beneficial microbial activity near roots.

A field study in quinoa investigated P. putida together with Bacillus subtilis alongside mineral fertilization, demonstrating a practical route for incorporating these bacteria into an irrigated cropping system. [5]

Biological Inputs and Custom Consortia

IndoGulf BioAg can assess P. putida for custom formulations involving other beneficial microorganisms, including Bacillus species, mycorrhizal fungi and Trichoderma.

Formulation development aligns the selected microorganisms with the crop, delivery method and intended product specification. Mycorrhizal components should be selected for crops that form the relevant fungal association.

Humic Products, Seaweed Extracts and Organic Amendments

These inputs can be considered within the wider crop program. Our team can advise how to schedule biological applications alongside soil conditioners and biostimulants, and which combinations are suitable for a prepared formulation or application mixture.

Crop-Protection Inputs

For treated seed or programs using fungicides and bactericides, IndoGulf BioAg provides product-specific guidance on suitable combinations and application timing.

Contact our team to integrate P. putida into your existing crop program or develop a custom microbial product.

Dosage & Application

Seed Coating and Seed Treatment

Apply a seed-treatment formulation evenly using the specified slurry or binder system. Maintain uniform coverage and seed flowability, protect treated seed from excessive heat, and follow the recommended treatment-to-sowing interval.

For commercial seed-treatment development, our team can support formulation selection and the assessment of coating compatibility, microbial survival and on-seed stability.

Seedling Root Treatment

A root-treatment formulation places the inoculant around transplant roots before planting. Prepare and apply it according to its product instructions, then transplant promptly while protecting roots from drying.

Soil and Root-Zone Application

Apply near the planting row, planting hole or active root zone, according to the selected product. Maintain suitable soil moisture to support establishment after application.

Irrigation and Fertigation

An irrigation-suitable formulation can deliver the inoculant to the root zone. Follow the product instructions for preparation, dilution and injection timing. Field research has evaluated irrigation delivery of a P. putida–B. subtilis combination. [5]

Practical Guidance

Use the product rate for the selected formulation and application route.
Prepare with water that meets the product’s requirements.
Protect the product and prepared mixture from excessive heat and direct sunlight.
Follow the specified timing for co-applied fertilizers, crop-protection products and other inputs.

FAQ

What is Pseudomonas putida?

Pseudomonas putida is a soil-associated bacterium. Agricultural research investigates selected strains for phosphate solubilization, root interactions and plant growth promotion. [1, 6]

Is Pseudomonas putida Gram-positive or Gram-negative?

It is Gram-negative. Its cells are rod-shaped, and the species does not form the resistant endospores characteristic of many Bacillus species.

Is Pseudomonas putida a phosphate-solubilizing bacterium?

Selected strains are. Their activity can release phosphate from poorly soluble mineral sources. This makes phosphorus availability an appropriate focus for agricultural formulation development.

Can Pseudomonas putida be used with fertilizer?

Yes, it can be incorporated into a fertilized crop program. Use the selected formulation’s instructions to determine whether to apply it separately or in an approved mixture. Field research has evaluated a P. putida–B. subtilis inoculant alongside mineral fertilization.

Can it be combined with mycorrhizal fungi or other beneficial microbes?

These are options for custom formulation development. IndoGulf BioAg can help select microbial combinations and application approaches for the intended crop and growing system.

Which crops have been studied?

Examples include maize in greenhouse research, mustard under greenhouse salinity treatments, and quinoa in a field trial involving co-inoculation with B. subtilis. These studies support crop-specific development rather than a single recommendation for every crop.

Can Pseudomonas putida improve crop yield?

Its root- and nutrient-related functions provide a rationale for evaluating crop productivity. A consistent commercial yield response must be established for the particular strain, formulation and crop program; biomass or root-growth responses alone do not establish harvested yield.