Gluconacetobacter Diazotrophicus Manufacturer & Exporter | Nitrogen Fixing Bacteria | Microbial Species

Gluconacetobacter diazotrophicus

Gluconacetobacter diazotrophicus is a beneficial bacterium used in agriculture for its association with sugarcane and other crops, where it fixes nitrogen and enhances plant growth and productivity.

Strength

1 x 10⁸ CFU per gram / 1 x 10⁹ CFU per gram / 1 x 10¹⁰ CFU per gram

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Benefits

Stress Tolerance

Gluconacetobacter diazotrophicus synthesizes stress-protective compounds like osmoprotectants and antioxidants, helping plants tolerate various stresses like drought and salinity.

Enhanced Plant Growth

It promotes plant growth through the production of plant growth-promoting substances such as auxins and gibberellins, stimulating root and shoot development.

Nitrogen Fixation

Gluconacetobacter diazotrophicus fixes atmospheric nitrogen into ammonia, providing a direct nitrogen source to the host plant, which enhances plant growth and reduces fertilizer dependency.

Biofertilizer

It serves as a biofertilizer, enhancing soil fertility and nutrient availability by solubilizing phosphates and other minerals, improving overall soil health.

Scientific References

Gluconacetobacter diazotrophicus has been extensively studied with over 200 research publications demonstrating its nitrogen fixation capabilities, plant growth promotion effects, and successful application in various crops including sugarcane, rice, tomato, and maize. frontiersin+2

"Gluconacetobacter diazotrophicus: A Model for Plant Growth Promotion"

URL: https://pubmed.ncbi.nlm.nih.gov/10066818/
"Biological nitrogen fixation in sugarcane by Gluconacetobacter diazotrophicus"

URL: https://www.sciencedirect.com/science/article/pii/S0925521409002299
"Genomic insights into nitrogen fixation and plant association of Gluconacetobacter diazotrophicus PA1 5"

URL: https://www.frontiersin.org/articles/10.3389/fmicb.2014.00093/full
"The Methylotrophic, Nitrogen-Fixing Bacterium Gluconacetobacter diazotrophicus Interacts with the
Endophytic Environment of Sugarcane"
URL: https://journals.asm.org/doi/10.1128/aem.63.11.4661-4667.1997
"Plant Growth Promotion and Biocontrol Activity of Gluconacetobacter diazotrophicus Isolated from Sugarcane"
URL: https://www.scielo.br/j/bjm/a/ZjvjFSHRz6YcFtzQ9CvFhmG/?lang=en

Mode of Action

Biological Nitrogen Fixation: Utilizes the nitrogenase enzyme complex (NifDK) to convert atmospheric nitrogen into ammonia under microaerobic conditions, with protective mechanisms including CowN protein that prevents carbon monoxide inhibition and upregulated antioxidant pathways to control reactive oxygen species.

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Plant Growth Promotion: Produces indole-3-acetic acid (IAA) through the indole pyruvate pathway, synthesizes gibberellins A1 and A3, and solubilizes insoluble phosphates through gluconic acid production via pyrroloquinoline quinone-linked glucose dehydrogenase activity.

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Endophytic Colonization: Establishes in plant apoplastic spaces through root emergence sites, root hairs, and root tips without forming nodules, allowing direct nutrient transfer to host plants while activating plant defense mechanisms against pathogens.

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Additional Info

Recommended Crops: Cereals, Millets, Pulses, Oilseeds, Fibre Crops, Sugar Crops, Forage Crops, Plantation crops, Vegetables, Fruits, Spices, Flowers, Medicinal crops, Aromatic Crops, Orchards, and Ornamentals.

Compatibility: Compatible with Bio Pesticides, Bio Fertilizers, and Plant growth hormones but not with chemical fertilizers and chemical pesticides.

Shelf Life: Stable within 1 year from the date of manufacturing.

Packing: We offer tailor-made packaging as per customers' requirements.

Dosage & Application

Seed Treatment: Prepare a mixture of 2.5 kg of Gluconacetobacter Diazotrophicus with 200 liters of water and soak seeds for 30 minutes before planting. Do not store treated seeds for more than an hour.

Soil Treatment: Mix 3-5 kg per acre of Gluconacetobacter Diazotrophicus with organic manure or fertilizers. Incorporate into the soil during planting or sowing.

Irrigation: Mix 3 kg per acre of Gluconacetobacter Diazotrophicus in water and apply through drip lines.

FAQ

What is the use of Gluconacetobacter diazotrophicus?

Primary uses include biological nitrogen fixation (reducing fertilizer dependency by up to 50%), plant growth promotion through hormone production, phosphate solubilization, and enhancing plant stress tolerance under drought and nutrient-limited conditions.

(pubmed.ncbi.nlm.nih+2)

What are the benefits of Gluconacetobacter diazotrophicus?

Key benefits include increased plant biomass (up to 92% dry weight increase), enhanced root and shoot development, improved nitrogen uptake efficiency, phosphate availability enhancement, production of growth-promoting hormones (auxins, gibberellins), and activation of plant defense responses against pathogens.

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Why is Gluconacetobacter diazotrophicus preferred for inoculating sugarcane?

Originally discovered in Brazilian sugarcane, it naturally colonizes sugarcane tissues as an endophyte, demonstrates high compatibility with sugarcane physiology, can provide up to 80% of plant nitrogen requirements through biological fixation, and has proven effectiveness in increasing sugarcane yield and reducing fertilizer costs in field trials.

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