Bacillus Tequilensis beneficial bacteria for Agriculture | Manufacturer & Exporter | Biofungicides | Microbial Species

Bacillus tequilensis

Bacillus tequilensis is a Gram-positive, endospore-forming bacterium with significant roles in agriculture and biotechnology. It enhances plant growth via phytohormone synthesis, nutrient solubilization, and antimicrobial…

Strength

1 x 10⁸ CFU per gram / 1 x 10⁹ CFU per gram

Product Enquiry

Benefits

Soil Health Improvement

Improves soil health by promoting organic matter decomposition and nutrient cycling, contributing to sustainable agriculture practices.

Biocontrol Agent

Bacillus tequilensis acts as a biocontrol agent, suppressing plant pathogens through the production of antimicrobial compounds.

Stress Tolerance

Helps plants withstand various environmental stresses, including drought and salinity, by inducing stress tolerance mechanisms.

Plant Growth Promotion

Enhances plant growth by producing growth-promoting substances such as phytohormones and siderophores, facilitating nutrient uptake.

FAQ

Q: What is Bacillus tequilensis?

A: Bacillus tequilensis is a species of bacteria belonging to the genus Bacillus. It's known for its diverse metabolic capabilities and its potential applications in various fields, particularly in agriculture as a biocontrol agent.

Q: What are the main modes of action of Bacillus tequilensis?

A: The primary modes of action include: Production of Antimicrobial Substances: Synthesizing compounds like lipopeptides, bacteriocins, volatile organic compounds (VOCs), and lytic enzymes that directly inhibit or kill pathogens. Induction of Plant Resistance (ISR): Triggering the plant's own defense mechanisms to become more resistant to diseases. Competition: Outcompeting pathogenic microorganisms for nutrients and space.

Biofilm Formation: Creating a protective barrier on plant roots against pathogen invasion.

Q: How does Bacillus tequilensis produce antimicrobial substances?

A: B. tequilensis can produce a range of compounds, including: Lipopeptides and biosurfactants: Disrupting pathogen cell membranes. Bacteriocins: Inhibiting the growth of other bacteria. Volatile Organic Compounds (VOCs): Exhibiting antifungal activity. Lytic Enzymes (e.g., chitinase, protease): Degrading pathogen cell walls. * Other Antibiotics: Novel compounds with antimicrobial properties.

Q: How does Bacillus tequilensis induce plant resistance?

A: It triggers the plant's defense system through mechanisms such as: Activation of the phenylpropanoid pathway: Leading to the production of defense-related compounds. Enhancement of defense-related enzyme activities: Boosting enzymes involved in plant immunity. * Stimulation of plant growth and development: Indirectly contributing to resistance through improved plant health.

Q: Can Bacillus tequilensis help plants grow?

A: Yes, some strains can produce indole-3-acetic acid (IAA), a plant hormone that promotes root growth and overall plant vigor. This can indirectly enhance the plant's ability to withstand stress, including pathogen attacks.

Q: What makes Bacillus tequilensis a good candidate for biocontrol?

A: Its multiple modes of action, including direct antimicrobial activity and the ability to induce plant resistance, make it effective against a range of plant pathogens. Additionally, Bacillus species are generally known for their ability to colonize the rhizosphere and their relative safety.

Q: Is Bacillus tequilensis safe for the environment?

A: When used as a biocontrol agent, Bacillus tequilensis is generally considered environmentally friendly as it offers a more sustainable alternative to synthetic pesticides. However, specific formulations and application methods should always be evaluated for their environmental impact.

Q: Where can Bacillus tequilensis be found?

A: Bacillus species are widely distributed in nature and can be found in soil, water, and associated with plants. Bacillus tequilensis was initially isolated from a tequila fermentation process, hence its name.

Q: Are there different strains of Bacillus tequilensis with varying modes of action?

A: Yes, different strains within the Bacillus tequilensis species can exhibit variations in their metabolic capabilities and the specific antimicrobial compounds they produce, as well as their effectiveness in inducing plant resistance.

Q: How is Bacillus tequilensis applied in agriculture?

A: It can be applied through various methods, including seed treatments, soil drenching, and foliar sprays, depending on the target pathogen and the crop.

Scientific References

Title: The Genus Bacillus: Applications and Biotechnological Potential
https://www.intechopen.com/chapters/76175

Relevance: Provides a broad overview of the biotechnological potential of various Bacillus species, including their roles in plant growth promotion, biocontrol, and bioremediation. Offers context for the broader impact of Bacillus in sustainable agriculture and environmental management.
Title: Bacillus tequilensis as a broad-spectrum antifungal agent against phytopathogenic fungi https://pubmed.ncbi.nlm.nih.gov/32358811/

Relevance: This study details the antifungal properties of Bacillus tequilensis, showcasing the effectiveness of this bacterial strain in combating various plant pathogens. This provides a scientific basis for incorporating it into biocontrol products.
Title: Draft Genome Sequence of Bacillus tequilensis Strain ZSB20, an Endophytic Diazotroph with Antimicrobial Activity, Isolated from Grape Roots

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5664934

Relevance: Provides genomic evidence for the diazotrophic (nitrogen-fixing) and antimicrobial capabilities of Bacillus tequilensis, validating its role as a beneficial endophyte for promoting plant health.
Title: Plant Growth Promoting Potential of Bacillus tequilensis and Bacillus amyloliquefaciens Isolated from Saline Soil
https://www.researchgate.net/publication/344037808_Plant_Growth_Promoting_Potential_of_Bacillus_tequilensis_and_Bacillus_amyloliquefaciens_Isolated_from_Saline_Soil

Relevance: It shows the isolation of B. tequilensis from saline soil and its ability to promote plant growth under salt stress conditions, this study supports its use in salinity management and improving crop yields in salt-affected areas.
Title: Characterization of the Biosurfactant Produced by Bacillus tequilensis and Its Application in Enhanced Oil Recovery.
https://www.proquest.com/openview/4f200c3b1fdc247c90d566d7d4a03f7c/1?pq-origsite=gscholar&cbl=18750&diss=y

Relevance: This article characterizes the biosurfactant produced by B. tequilensis and explores its application in enhanced oil recovery. It offers insight into the surface-active properties of B. tequilensis, such as reducing surface and interfacial tension.

Mode of Action

Bacillus tequilensis exhibits a variety of modes of action, primarily centered around antimicrobial activity and the induction of plant resistance. Here's a breakdown of the key mechanisms:

1. Production of Antimicrobial Substances:

B. tequilensis can produce various secondary metabolites with antimicrobial properties. These can include:
- Lipopeptides and biosurfactants: These compounds can disrupt the cell membranes of pathogenic fungi and bacteria, leading to leakage of cellular contents and cell death. Examples include iturins and fengycins.
- Bacteriocins: These are proteinaceous toxins produced by bacteria to inhibit the growth of similar or closely related bacterial strains.
- Volatile Organic Compounds (VOCs): Certain VOCs produced by B. tequilensis have demonstrated antifungal activity by inhibiting spore formation and germination and altering the cell morphology of pathogens.
- Enzymes: Production of lytic enzymes like chitinase, protease, and cellulase can degrade the cell walls of fungal pathogens.
- Other Antibiotic Compounds: Novel antibiotic agents like pyrrolo[1,2-a]pyrazine-1,4-dione,hexahydro, have been isolated from B. tequilensis with activity against multi-drug resistant bacteria.

2. Induction of Plant Resistance (Induced Systemic Resistance - ISR):

B. tequilensis can trigger defense mechanisms in plants, making them more resistant to pathogen attacks. This can involve:
- Activation of the phenylpropanoid pathway: This pathway leads to the synthesis of various defense-related compounds like lignin and phenolic compounds, which strengthen plant cell walls and have antimicrobial properties.
- Enhancement of defense-related enzyme activities: B. tequilensis can induce the activity of enzymes such as phenylalanine ammonia-lyase (PAL), cinnamate 4-hydroxylase (C4H), 4-coumarate CoA ligase (4CL), polyphenol oxidase (PPO), superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD). These enzymes play crucial roles in plant defense responses.
- Stimulation of plant growth and development: Some strains of B. tequilensis can produce indole-3-acetic acid (IAA), a plant hormone that promotes root growth and overall plant vigor, indirectly contributing to disease resistance.

3. Competition:

B. tequilensis can compete with pathogenic microorganisms for essential nutrients and space in the plant rhizosphere or on plant surfaces, limiting pathogen colonization and growth.

4. Biofilm Formation:

The ability of Bacillus species to form biofilms on plant roots can create a protective barrier against pathogen invasion and further infection.

Additional Info

Target pests: Fusarium wilt of tomato, leaf-spot disease of banana plants

Recommended Crops: Tomato, banana, rice.

Compatibility: Compatible with Bio Pesticides, Bio Fertilizers, and Plant growth hormones but not with chemical fertilizers and chemical pesticides.

Shelf Life: Stable within 1 year from the date of manufacturing.

Packing: We offer tailor-made packaging as per customers' requirements.

Dosage & Application

The water-soluble powder formulation of Bacillus tequilensis is engineered for ease of use and maximum efficacy across a wide range of environmental and agricultural applications. Leveraging its robust biosurfactant production, cellulolytic activity, and broad-spectrum biocontrol potential, B. tequilensis is an ideal choice for bioremediation, pest control, organic matter recycling, and sustainable crop management.

General Guidelines

Preparation:Dissolve the required quantity of B. tequilensis powder in clean, non-chlorinated water. Avoid chlorinated water, as it may reduce bacterial viability and activity. Use a container or tank with adequate mixing to ensure complete dissolution.

Activation Time:Allow the solution to rest for 15–30 minutes after mixing. This activates the microbial population and optimizes performance upon application.

Application Timing:Apply early in the morning or late in the afternoon to minimize exposure to high temperatures and UV radiation, both of which can diminish bacterial efficacy.

Dosage Recommendations

1. Bioremediation of Soil and Water

Target: Hydrocarbons, heavy metals, and organic pollutants.

Dosage: Dissolve 1–2 kg of powder in 200–400 liters of water per hectare for soil application. For water bodies, use 5–10 g per cubic meter of contaminated water.
Application: Spray uniformly over contaminated soil or introduce directly into the polluted water body. B. tequilensis produces biosurfactants that enhance the breakdown and bioavailability of hydrocarbons and other pollutants .
Frequency: Reapply every 3–4 weeks until remediation targets are met.

2. Pest and Disease Biocontrol in Agriculture

Target: Soil-borne pathogens, fungal diseases, and certain pests.

Dosage: Dissolve 500 g of powder in 100 liters of water per hectare.
Application:
- Foliar Spray: Apply evenly over plant foliage to suppress fungal and bacterial pathogens.
- Soil Drench: Apply directly to the root zone to control soil-borne diseases and enhance root health.
Frequency: Reapply every 2–3 weeks or as needed based on disease pressure. B. tequilensis is effective against a broad spectrum of plant pathogens, including Magnaporthe oryzae, Phytophthora nicotianae, Verticillium dahliae, and othes.

3. Nutrient Cycling and Organic Matter Decomposition

Target: Soil enrichment, compost acceleration, and nutrient recycling.

Dosage: Dissolve 1 kg of powder in 200 liters of water per hectare.
Application: Apply as a soil drench or through fertigation systems. B. tequilensis exhibits strong cellulolytic activity, accelerating the breakdown of plant residues and improving soil fertility
Frequency: Apply at the start of the growing season and repeat every 4–6 weeks for sustained soil health benefits.

4. Hydrocarbon and Industrial Waste Biodegradation

Target: Hydrocarbons and organic waste in soil or industrial effluents.

Dosage: Dissolve 1–2 kg of powder in 200–400 liters of water per hectare.
Application: Spray over contaminated sites or introduce into waste streams. The biosurfactant-producing capacity of B. tequilensis enhances the emulsification and breakdown of recalcitrant pollutants.
Frequency: Reapply every 4 weeks until remediation is complete.

5. Abiotic Stress Alleviation in Crops

Target: Salinity and drought stress in sensitive crops.

Dosage: 500 g–1 kg per hectare, dissolved in adequate water.
Application: Soil drench or seed treatment. B. tequilensis has demonstrated efficacy in improving crop growth, nutrient uptake, and physiological resilience under saline and drought conditions, notably in rice and other cereals.
Frequency: Apply at planting and repeat at key crop stages.

Best Practices & Additional Notes

For maximum biocontrol efficacy, consider integrating B. tequilensis with compatible carriers (e.g., biochar) or in consortia with other Bacillus species to enhance disease suppression and soil health10.
Thermal and pH Stability: B. tequilensis metabolites remain active under a range of temperatures and acidic conditions, making it suitable for diverse environments.
Environmental Safety: B. tequilensis is non-toxic to plants, animals, and humans when used as directed, supporting sustainable and eco-friendly management practices.

Summary:Bacillus tequilensis is a versatile, science-backed microbial solution for bioremediation, crop protection, soil fertility, and stress mitigation. Its robust biosurfactant and enzyme production, broad-spectrum pathogen suppression, and adaptability to challenging environments make it a valuable tool for modern agriculture and environmental management.

For technical support or custom application protocols, please contact us.